Mechanisms
Sci Rep.2012;2:314. doi: 10.1038/srep00314. Epub 2012 Mar 15.
Stilbene derivatives promote Ago2-dependent tumour-suppressive microRNA activity.
Hagiwara K1, Kosaka N, Yoshioka Y, Takahashi RU, Takeshita F, Ochiya T.
Abstract
It is well known that natural products are a rich source of compounds for applications in medicine, pharmacy, and biology. However, the exact molecular mechanisms of natural agents in human health have not been clearly defined. Here, we demonstrate for the first time that the polyphenolic phytoalexin resveratrol promotes expression and activity of Argonaute2 (Ago2), a central RNA interference (RNAi) component, which thereby inhibits breast cancer stem-like cell characteristics by increasing the expression of a number of tumour-suppressive miRNAs, including miR-16, -141, -143, and -200c. Most importantly, resveratrol-induced Ago2 resulted in a long-term gene silencing response. We also found that pterostilbene, which is a natural dimethylated resveratrol
PMID: 22423322
BMC Med Genomics.2008 Mar 20;1:7. doi: 10.1186/1755-8794-1-7.
Identification of molecular pathways affected by pterostilbene, a natural
Pan Z1
Abstract
BACKGROUND: Pterostilbene, a naturally occurring phenolic compound produced by agronomically important plant genera such as Vitis and
METHODS: S. cerevisiae strain S288C was exposed to pterostilbene at the IC50 concentration (70 muM) for one generation (3 h). Transcript profiling experiments were performed on three biological replicate samples using the Affymetrix GeneChip Yeast Genome S98 Array. The data were analyzed using the statistical methods available in the GeneSifter microarray data analysis system. To validate the results, eleven differentially expressed genes were further examined by quantitative real-time RT-PCR, and S. cerevisiae mutant strains with deletions in these genes were analyzed for altered sensitivity to pterostilbene.
RESULTS: Transcript profiling studies revealed that pterostilbene exposure significantly down-regulated the expression of genes involved in methionine metabolism, while the expression of genes involved in mitochondrial functions, drug detoxification, and transcription factor activity were significantly up-regulated. Additional analyses revealed that a large number of genes involved in lipid metabolism were also affected by pterostilbene treatment.
CONCLUSION: Using transcript profiling, we have identified the cellular pathways targeted by pterostilbene, an analog of resveratrol. The observed response in lipid metabolism genes is consistent with its known hypolipidemic properties, and the induction of mitochondrial genes is consistent with its demonstrated role in apoptosis in human cancer cell lines. Furthermore, our data show that pterostilbene has a significant effect on methionine metabolism, a previously unreported effect for this compound.
PMID: 18366703
J Gastrointest Surg.2012 Jun;16(6):1136-43. doi: 10.1007/s11605-012-1869-7. Epub 2012 Mar 27.
Genomic analysis of pterostilbene predicts its antiproliferative effects against pancreatic cancer in vitro and in vivo.
McCormack DE1
Abstract
BACKGROUND: To investigate the inhibitory role of pterostilbene in pancreatic cancer, we conducted a genomic analysis of pterostilbene-treated pancreatic cancer cells. We also investigated the effect of pterostilbene upon the carcinogenic markers, manganese superoxide dismutase, cytochrome C, Smac/DIABLO, and STAT3 phosphorylation in vitro. The antiproliferative effects of pterostilbene were further evaluated in an in vivo model.
METHODS: Pancreatic cancer cells were treated with pterostilbene and evaluated with DNA microarray analysis. Pterostilbene-treated cells were analyzed for cytochrome C, Smac/DIABLO, manganese superoxide dismutase (MnSOD)/antioxidant activity, and STAT3 phosphorylation using ELISA. Data were statistically analyzed using ANOVA. Pterostilbene was then administered to nude mice for 8 weeks, and tumor growth rates were recorded and statistically analyzed.
RESULTS: Microarray analysis of pterostilbene-treated cells revealed upregulation of pro-apoptosis genes. In vitro, pterostilbene treatment altered levels of phosphorylated STAT3, MnSOD/antioxidant activity, cytochrome C, and Smac/DIABLO. In nude mice, oral pterostilbene inhibited tumor growth rates.
CONCLUSION: Pterostilbene alters gene expression in pancreatic cancer and increases the antiproliferative markers cytochrome C, Smac/DIABLO, and MnSOD/antioxidant activity. It was also shown to inhibit phosphorylated STAT3, a marker of accelerated tumorigenesis, and decrease pancreatic tumor growth in vivo. Further studies are warranted to elucidate the effects of pterostilbene in humans.
PMID: 22450950
Mol Nutr Food Res.2013 May;57(5):886-95. doi: 10.1002/mnfr.201200715. Epub 2013 Feb 18.
Invadopodia-associated proteins blockade as a novel mechanism for 6-
Hong BH1
Abstract
SCOPE: Invadopodia are actin-rich membrane protrusions of tumor cells that are thought to initiate the local migration and invasion during cancer metastasis. The blockade of invadopodia-associated proteins has been reported as a promising approach for
METHODS AND RESULTS: By wound-healing, transwell, and gelatin zymography assays, we found that 6-
CONCLUSION: These data suggest that the repression of these factors might affect the maturation of invadopodia, inhibiting the metastasis of MDA-MB-231 cells. In conclusion, the present study demonstrates for the first time that 6-
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
PMID: 23417847
Chem Biol Interact.2013 Nov 25;206(2):175-85. doi: 10.1016/j.cbi.2013.09.013. Epub 2013 Sep 25.
Scavenging of hydroxyl radical by resveratrol and related natural stilbenes after hydrogen peroxide attack on DNA.
Rossi M1,Caruso F, Antonioletti R, Viglianti A, Traversi G, Leone S, Basso E, Cozzi R.
Abstract
Resveratrol (3,5,4′-trihydroxystilbene) is of interest due to its role in
Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.
KEYWORDS: DNA protection; Hydrogen peroxide; Hydroxyl radical; Pterostilbene; Resveratrol
PMID: 24075811
Curr Pharm Biotechnol.2014;14(12):1027-35.
Pterostilbene as a potential novel telomerase inhibitor: molecular docking studies and its in vitro evaluation.
Tippani R,Prakhya LJ, Porika M, Sirisha K, Abbagani S, Thammidala C1.
Abstract
Pterostilbene is a naturally occurring dimethyl ether analog of resveratrol identified in several plant species. Telomerase is important in tumor initiation and cellular immortalization. Given the striking correlations between telomerase activity and proliferation capacity in tumor cells, telomerase had been considered as a potentially important molecular target in cancer therapeutics. Molecular docking studies were performed on pterostilbene with the crystal structure of telomerase (3DU6). Pterostilbene was evaluated for its in vitro cytotoxicity in
PMID: 24433502
Bioorg Med Chem Lett.2014 Feb 15;24(4):1176-9. doi: 10.1016/j.bmcl.2013.12.115. Epub 2014 Jan 7.
Urokinase-type plasminogen activator expression and Rac1/WAVE-2/Arp2/3 pathway are blocked by pterostilbene to suppress cell migration and invasion in MDA-MB-231 cells.
Ko HS1
Abstract
Breast cancer is the most common malignancy among females, and cancer invasion and metastasis are the leading causes of cancer death in breast cancer patients. Pterostilbene, a naturally occurring
Copyright © 2014 The Authors. Published by Elsevier
KEYWORDS: Arp2/3; Invasion; MDA-MB-231 cells; Migration; NF-κB; Pterostilbene; Rac1; WAVE; uPA
PMID: 24440300
J Pharmacol Sci.2014;126(3):216-29. Epub 2014 Oct 21.
Involvement of the Nrf2 pathway in the regulation of pterostilbene-induced apoptosis in HeLa cells via ER stress.
Zhang B1, Wang XQ, Chen HY, Liu BH.
Abstract
Among the various cancer cell lines, HeLa cells were found to be sensitive to pterostilbene (Pte), a compound that is enriched in small fruits such as grapes and berries. However, the mechanism involved in the cytotoxicity of Pte has not been fully characterized. Using biochemical and free radical biological experiments in vitro, we identified the pro-apoptotic profiles of Pte and evaluated the level of redox stress-triggered ER stress during HeLa cell apoptosis. The data showed a strong dose-response relationship between Pte exposure and the characteristics of HeLa apoptosis in terms of changes in apoptotic morphology, DNA fragmentation, and activated caspases in the intrinsic apoptotic pathway. During drug exposure, alterations in the intracellular redox homeostasis that favor oxidation
PMID: 25341683
Int Immunopharmacol.2015 Sep;28(1):10-21. doi: 10.1016/j.intimp.2015.05.003. Epub 2015 May 13.
Understanding the mode of action of a pterostilbene derivative as
Nikhil K1,Sharan S1, Palla SR2, Sondhi SM2, Peddinti RK2, Roy P3.
Abstract
Inflammatory response plays an important role not only in the normal
Copyright © 2015 Elsevier B.V. All rights reserved.
KEYWORDS: Anti-inflammatory; Carrageenan; Lipopolysaccharide; NFκB; Pterostilbenederivative; RAW 264.7 macrophage
PMID: 25981112
Mol Carcinog.2017 Mar;56(3):1117-1126. doi: 10.1002/mc.22578. Epub 2016 Oct 24.
The resveratrol
Traversi G1,Fiore M2, Percario Z1, Degrassi F2, Cozzi R1.
Abstract
Natural compounds are extensively studied for their potential use in traditional and non-traditional medicine. Several natural and synthetic Resveratrol
© 2016 Wiley Periodicals, Inc.
KEYWORDS: apoptosis;
PMID: 27739192