Bladder Cancer
Mol Nutr Food Res.2010 Dec;54(12):1819-32. doi: 10.1002/mnfr.201000067.
Pterostilbene induces autophagy and apoptosis in sensitive and chemoresistant human bladder cancer cells.
Abstract
SCOPE: Bladder cancer is one of the most common malignancies in the world. The majority of bladder cancer deaths are due to unresectable lesions that are resistant to chemotherapy. Pterostilbene(PT), a naturally occurring phytoalexin, possesses a variety of pharmacologic activities, including antioxidant, cancer prevention activity and cytotoxicity to many cancers. We found that PT effectively inhibits the growth of sensitive and chemoresistant human bladder cancer cells by inducing cell cycle arrest, autophagy
METHODS AND RESULTS: Autophagy occurred at an early stage and was observed through the formation of acidic vesicular organelles (the marker for autophagy) and microtubule-associated protein 1 light chain 3-II production. Apoptosis occurred at a later stage and was detected by Annexin V and 4′,6-diamidino-2-phenylindole staining. PT-induced autophagy was triggered by the inhibition of active human protein kinase/the mammalian TOR/p70S6K pathway and activation of
CONCLUSION: This is the first study to demonstrate that PT causes autophagy in cancer cells and suggests that PT could serve as a new and promising agent for the treatment of sensitive and chemoresistant bladder cancer cells.
Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
PMID: 20603834