Lung Cancer
J Surg Res.2010 Jun 1;161(1):18-22. doi: 10.1016/j.jss.2009.06.027. Epub 2009 Jul 21.
Pterostilbene inhibits lung cancer through induction of apoptosis.
Schneider JG1,Alosi JA, McDonald DE, McFadden DW.
Abstract
BACKGROUND: Lung cancer remains the leading cause of cancer mortality in the United States. Resveratrol is a potent antioxidant found in grapes that inhibits several types of cancer, including lung cancer. Herein, we investigated the effects of pterostilbene, an analog of resveratrol found in blueberries, on lung cancer, in vitro. We hypothesized that pterostilbene would inhibit lung cancer cell growth in vitro by a pro-apoptotic mechanism.
METHODS: Two lung cancer cell lines (NCI-H460 and SK-MES-1) were cultured using standard techniques. Cells were treated with increasing doses of pterostilbene(10-100 microM). Cell viability was measured at 24, 48, and 72h using
RESULTS: Pterostilbene significantly decreased cell viability in lung cancer cells in a concentration- and time-dependent manner (P<0.001). Concentrations greater than 20
CONCLUSIONS: Pterostilbene inhibits growth via apoptosis induction in vitro. Further in vitro mechanistic studies and in vivo experiments are warranted to determine the potential role for pterostilbene in lung cancer treatment or prevention.
Copyright (c) 2010 Elsevier Inc. All rights reserved.
PMID: 20031166
PLoS One.2013 May 3;8(5):e62652. doi: 10.1371/journal.pone.0062652. Print 2013.
Pterostilbene exerts antitumor activity via the Notch1 signaling pathway in human lung adenocarcinoma cells.
Yang Y1, Yan X, Duan W, Yan J, Yi W, Liang Z, Wang N, Li Y, Chen W, Yu S, Jin Z, Yi D.
Abstract
Although pterostilbene (PTE) has been shown to have potent antitumor activities against various cancer types, the molecular mechanisms of these activities remain unclear. In this study, we investigated the antitumor activity of PTE against human lung adenocarcinoma in vitro and in vivo and explored the role of the Notch1 signaling pathway in this process. PTE treatment resulted in a dose- and time-dependent decrease in the viability of A549 cells. Additionally, PTE exhibited strong antitumor activity, as evidenced not only by a reduced mitochondrial membrane potential (MMP) and a decreased intracellular glutathione content but also by increases in the apoptotic index and the level of reactive oxygen species (ROS). Furthermore, PTE treatment induced the activation of the Notch1 Intracellular Domain (NICD) protein and activated Hes1. DAPT (a
PMID: 23671619
Toxicol Sci.2014 Jan;137(1):65-75. doi: 10.1093/toxsci/kft238. Epub 2013 Oct 23.
A combination of pterostilbene with autophagy inhibitors exerts efficient apoptotic characteristics in both chemosensitive and chemoresistant lung cancer cells.
Hsieh MJ1
Abstract
The emergence of multidrug resistance (MDR), meaning that cancer cells develop simultaneous resistance to different drugs, has limited the clinical efficacy and application of chemotherapy. Pterostilbene, a naturally occurring phytoalexin exerts a variety of pharmacologic activities, including cancer prevention, cytotoxicity, and antioxidant activity. In this study, results proved the capability of pterostilbene to effectively inhibit the cell viability of docetaxel-induced MDR human lung cancer cell lines through cell cycle arrest and apoptosis. Meanwhile, the observation of LC3-II production and formation of acidic vesicular organelles revealed
KEYWORDS: apoptosis; autophagy.; multidrug resistance; pterostilbene
PMID: 24154491
J Pharm Biomed Anal.2015 Oct 10;114:200-7.
UPLC-MS method for quantification of pterostilbene and its application to
Deng L1,Li Y2, Zhang X3, Chen B1, Deng Y1, Li Y1.
Abstract
A UPLC-MS method was developed for
Copyright © 2015 Elsevier B.V. All rights reserved.
KEYWORDS: Bioavailability;Pterostilbene; Tissue distribution; UPLC–MS
PMID: 26070162
PLoS One.2016 Sep 9;11(9):e0162335. doi: 10.1371/journal.pone.0162335. eCollection 2016.
ATM/CHK/p53
Lee H1, Kim Y2, Jeong JH1, Ryu JH1, Kim WY1.
Abstract
Among the many stilbenoids found in a variety of berries, resveratrol and pterostilbene are of particular interest given their potential for use in cancer therapeutics and prevention. We purified four stilbenoids from R.
PMID: 27612029
J Hematol Oncol.2017 Mar 21;10(1):72. doi: 10.1186/s13045-017-0441-z.
Pterostilbene prevents AKT-ERK axis-mediated polymerization of surface fibronectin on suspended lung cancer cells independently of apoptosis and suppresses metastasis.
Wang YJ1,2,3,4,Lin JF1, Cheng LH5, Chang WT5,6, Kao YH7, Chang MM6, Wang BJ1,8,9, Cheng HC10,11.
Abstract
BACKGROUND: Polymeric fibronectin (polyFN) assembled on suspended breast cancer cells is required for metastasis. Conceivably, drugs that target such polyFN may fight against cancer metastasis. While stilbene analogs trigger pro-apoptotic effect on attached cancer cells, whether they prevent polyFN assembly and metastasis of suspended cancer cells via an apoptosis-independent manner remains unexplored.
METHODS: We depleted suspended Lewis lung carcinoma (LLC) cells of polyFN by silencing the endogenous FN expression or pterostilbene(PS) to examine whether metastasis of lung cancer cells could thus be suppressed. We investigated whether PS regulates AKT-ERK signaling axis to suppress polyFN assembly in suspended LLC cells independently of apoptosis. We tested the therapeutic effects of orally administered PS against cancer metastasis.
RESULTS: Both FN-silencing and PS among the three stilbenoids indeed significantly reduced polyFN assembly and lung metastasis of suspended LLC cells in an apoptosis-independent manner. Mechanistically, PS-induced AKT phosphorylation (pAKT) and suppressed ERK phosphorylation (pERK) in suspended LLC cells, whereas pretreatment with a PI3K inhibitor, LY294002, effectively reduced pAKT, rescued pERK, and consequently reversed the PS-suppressed polyFN assembly on LLC cells; these pretreatment effects were then overturned by the ERK inhibitor U0126. Indeed, PS-suppressed lung metastasis was counteracted by LY294002, which was further overruled with U0126. Finally, we found that PS, when orally administered in experimental metastasis assays, both significantly prevented lung colonization and metastasis of LLC cells and reduced the already established tumor growth in the mouse lungs.
CONCLUSIONS: PS suppressed AKT/ERK-regulated polyFN assembly on suspended LLC cells and pulmonary metastasis. PS possesses potency in both preventing and treating lung metastasis of lung cancer cells in apoptosis-independent and apoptosis-dependent manners, respectively.
KEYWORDS: Fibronectin; PI3K/AKT/ERK signaling; Pericellular assembly; Pterostilbene; Pulmonary localization
PMID: 28327179